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KMID : 0380020040190030241
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 3 p.241 ~ p.244
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Automatic Measurement of Microbial Growth in Shake-Flask Culture using an Economic and Automated Flow Injection Analysis Apparatus
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Lee Hyeong-Choon
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Abstract
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An automated flow injection analysis apparatus manufactured economically was used in the automatic measurement of E. col growth in shake-flask culture of nutrient broth. The detailed measurement of whole growth was possible at intervals of 10 minutes by the automatic measurement system which adopted the sterilized nutrient broth as a carrier solution. Using distilled water as a carrier solution showed less accurate results than nutrient broth.
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KEYWORD
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Shake-flask culture, growth, automatic measurement, flow injection analysis
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